Effect of Vitamins (pyridoxine and nicotinic acid), Thiamine-Hcl and Myo-Inositol at Different Concentrations on Free Amino Acids and Indoles Content of Embryogeinic Callus of in vitro Date Oalm (Sakkoty and Bartamuda Cultivar)
Sherif F. El sharabasy, Hussein A. Bosila, Abdel-Aal W. B., Bayome M. Mansour, Abdel-Monem A. Bana
download PDFAbstract. The potential of using tissue culture technique for the production of some bioactive compounds since it allows the manipulation of the biosynthetic routes to increase the production and accumulation of specific compounds. This study was conducted to investigate the effect of vitamins (pyridoxine and nicotinic acid), thiamine-Hcl at different cocentrations (0.5, 1.0 & 2.0 mg/l) and myo-inositol at different concentrations (25, 50, and 100mg/l) at different cocentrations supplemented in MS basal nutrient medium of embryogenic callus of date palm on the production of secondary metabolites of amino acids and indoles. Tow egyption cultivars (Sakkoty and Bartamuda cultivars) of date palm were used. Pyridoxine concentration at 0.5mg/l was the most effective concentration in the production of amino acids and indoles from embryonic callus of the tow studied cultivars of date palm. Nicotinic acid at 0.5mg/l showed also the best results of production of amino acids and indoles from embryogenic callus of two cultivars. Acording to thiamine at 2mg/l concentration was the most effective in inducing the highest significant value of amino acids and indoles from embryonic callus of two cultivars of date palm. Myo-inositol concentration at 25mg/l produced the highest significant value of amino acids and indoles.
Keywords
vitamins, amino acid, indoles, embryogenic callus, tissue culture, date palm
Published online 4/20/2019, 9 pages
Copyright © 2019 by the author(s)
Published under license by Materials Research Forum LLC., Millersville PA, USA
Citation: Sherif F. El sharabasy, Hussein A. Bosila, Abdel-Aal W. B., Bayome M. Mansour, Abdel-Monem A. Bana, Effect of Vitamins (pyridoxine and nicotinic acid), Thiamine-Hcl and Myo-Inositol at Different Concentrations on Free Amino Acids and Indoles Content of Embryogeinic Callus of in vitro Date Oalm (Sakkoty and Bartamuda Cultivar), Materials Research Proceedings, Vol. 11, pp 244-252, 2019
DOI: https://doi.org/10.21741/9781644900178-20
The article was published as article 20 of the book By-Products of Palm Trees and Their Applications
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
References
[1] R. Muhaidat, M.A. Al-Qudah, O. Samir, J.H. Jacob,E. Hussein, I.N. Al-Tarawneh, E. Bsoul, S.T.Orabi, Phytochemical investigation and in vitro antibacterial activity of essential oils from Cleome droserifolia (Forssk.) Delile and C. trinervia Fresen.(Cleomaceae), South African J. Bot. 99 (2015) 21-8. https://doi.org/10.1016/j.sajb.2015.03.184
[2] M. Asif, Chemistry and antioxidant activity of plants containing some phenolic compounds,Chem. Int. 1 (2015), pp. 35-52.
[3] S. Gantait, M.M. El-Dawayati, J. Panigrahi, C. Labrooy, S.K. Verma, The retrospect and prospect of the applications of biotechnology in (Phoenix dactylifera L.), App. Microbial, Biotech. 102 (2018) 8229–8259. https://doi.org/10.1007/s00253-018-9232-x
[4] R. Al-Alawi, J. Al-Mashiqri, J. Al-Nadabi, B. Al-Shihi, Y. Baq, Date palm tree (Phoenix dactylifera L.) natural products and therapeutic options, Front Plant Sci 8 (2017) 1–12. https://doi.org/10.3389/fpls.2017.00845
[5] N.A Fadzliana, S. Rogayah, N.A. Shaharuddin, O.A. Janna, Addition of L-Tyrosine to Improve Betalain Production in Red Pitaya Callus, Pertanika J. Tropical Agr. Sci. 40-4 (2017) 521-532.
[6] A.P. Ling, S.L. Ong, H. Sobri, Strategies in enhancing secondary metabolites production in plant cell cultures, Med Aromat Plant Sci Biotechnol. 5 (2011) 94-101.
[7] A. Pérez, L. Nápoles, C. Carvajal, M. Hernandez, JC. Lorenzo, Effect of sucrose, inorganic salts, inositol, and thiamine on protease excretion during pineapple culture in temporary immersion bioreactors, In Vitro Cellular & Developmental Biology-Plant, 40-3 (2004) 311-316. https://doi.org/10.1079/ivp2004529
[8] A. Jacob, N. Malpathak, Manipulation of MS and B5 components for enhancement of growth and solasodine production in hairy root cultures of Solanum khasianum Clarke, Plant cell, tiss org cult. 80-3 (2005) 247-57. https://doi.org/10.1007/s11240-004-0740-2
[9] E.F. George, M.A. Hall, GJ De Klerk, The component of plant tissue culture media II. Organic additives, osmotic and pH effects and support system, In: Plant propagation by tissue culture the background (3rd Edn), Springer The Netherland 1 (2010) 115-174. https://doi.org/10.1007/978-1-4020-5005-3_4
[10] Z. E. Zayed, Enhanced Indirect Somatic Embryogenesis from Shoot-Tip Explants of Date Palm by Gradual Reductions of 2, 4-D Concentration, In Date Palm Biotechnology Protocols, Humana Press, New York 1 (2017) 77-88. https://doi.org/10.1007/978-1-4939-7156-5_7
[11] M.M. El-Dawayati, H.S. Ghazzawy, M. Munir, Somatic embryogenesis enhancement of date palm cultivar Sewi using different types of polyamines and glutamine amino acid concentration under in-vitro solid and liquid media conditions, Int J Biosci 12 (2018) 149-159. https://doi.org/10.12692/ijb/12.1.149-159
[12] T. Murashige, F. Skoog, A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant 15 (1962) 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
[13] H. Rosein, A modified ninhydrin coloremetric analysis for amino acids. Archives of Biochemistry and Biophysics 67 (1957) 10-15. https://doi.org/10.1016/0003-9861(57)90241-2
[14] P. Larsen, A. Harbo, S. Klungsour, T. Asheim, On the biogenesis of some indol compounds in Acetobacter xylinum, Physiologia Plantarum, 15 (1962) 552 – 655. https://doi.org/10.1111/j.1399-3054.1962.tb08058.x
[15] H.D. Danial, C.M. George, Peach seed dormancy in relation to endogenous inhibitors and applied growth substances, Journal of the American Society for Horticultural Science, 17 (1972) 651- 654.
[16] G.W. Snedecor, W.G. Cochran, Statisical Methods, Oxford and J.B.H. Publishing Co., 6th edition, 1980, pp. 507.
[17] M.I. Dias, M.J. Sousa, R.C. Alves, Ferreira IC. Exploring plant tissue culture to improve the production of phenolic compounds: A review, Industrial Crops and Products, 82 (2016) 9-22. https://doi.org/10.1016/j.indcrop.2015.12.016
[18] I. Smetanska, Production of secondary metabolites using plant cell cultures, In Food biotech Springer, Berlin, Heidelberg (2008)187-228.
[19] C.L. Marbun, N. Toruan-Mathius, C. Utomo, T. Liwang, Micropropagation of embryogenic callus of oil palm (Elaeis guineensis Jacq.) using temporary immersion system, Procedia Chemistry, 2015. https://doi.org/10.1016/j.proche.2015.03.018
[20] Bettendorff L. Thiamine, Handbook of Vitamins, 5th Edition. 2014, pp. 267-323.
[21] T. Thorpe, S.E.A Yeung, GJ. de Klerek, A. Robert, E.F. George, The component of plant tissue culture media II. Organic additives, osmotic and pH effects and support system, In: Plant propagation by tissue culture the background (3red Edn), Springer The Netherland 1 (2010) 115-174. https://doi.org/10.1007/978-1-4020-5005-3_4
[22] A.P. Ling, S.L. Ong, H. Sobri, Strategies in enhancing secondary metabolites production in plant cell cultures, Med Aromat Plant Sci Biotechnol. 5 (2011) 94-101.
