Effect of Murashige and Skoog Salts Strength Medium (MS) on Steroids Production and Total Amino Acids Content of Date Palm Embryonic Callus (Sakkoty and Bartamuda cultivar)
Hussein A.Bosila, Sherif F. El sharabasy, Abdel-Aal W. B., Bayome M. Mansour, Abdel-Monem A. Bana
download PDFAbstract. The potential to use tissue culture technique for the production of some bioactive compounds is immense, since it allows the manipulation of the biosynthetic routes to increase the production and accumulation of specific compounds. This study was conducted to investigate the effect of MS salt strength on steroids production and total amino acids content in embryonic callus cultures of two cultivars of date palm (Sakkoty and Bartamuda). embryonic callus explants were cultured on MS (Full), ¾ MS, ½ MS and ¼ MS), date was recorded every 6 weeks for three subculture. It obviously displays the superiority full MS over the three other investigated levels (¾ MS, ½ MS and ¼ MS) of steroids production (0.55, 0.38, 0.32 and 0.44 45mg/g dry weight respectively). Also the full MS level was the most effective of amino acids content (0.95 mg/g fresh weight). Bartamuda cv. was the superior of steroids production (0.45 mg/g dry weight) and amino acid content (1.13 mg/g fresh weight) compared with Sakkoty cvs.
Keywords
MS, steroids, amino acid, embryogenic callus, date palm
Published online 4/20/2019, 6 pages
Copyright © 2019 by the author(s)
Published under license by Materials Research Forum LLC., Millersville PA, USA
Citation: Hussein A.Bosila, Sherif F. El sharabasy, Abdel-Aal W. B., Bayome M. Mansour, Abdel-Monem A. Bana, Effect of Murashige and Skoog Salts Strength Medium (MS) on Steroids Production and Total Amino Acids Content of Date Palm Embryonic Callus (Sakkoty and Bartamuda cultivar), Materials Research Proceedings, Vol. 11, pp 229-234, 2019
DOI: https://doi.org/10.21741/9781644900178-18
The article was published as article 18 of the book By-Products of Palm Trees and Their Applications
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
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