Optimization of bioethanol production from papaya waste through fermentation using response surface methodology (RSM)
TAN Ching Mun, OH Pei Ching
download PDFAbstract. There is a growing pressure for the development of sustainable and environmental-friendly source of energy such as ethanol that could substitute the depleting fossil fuels. Papaya waste including papaya seed and papaya peel is one of the main fruit wastes in Southeast Asia which has great potential to be utilized as substrate for bioethanol production. In this study, papaya waste was fermented to produce bioethanol using Saccharomyces Cerevisiae. The effect of pH, temperature, and incubation time on bioethanol production was studied within the range of 3.0-6.0, 25-45°C and 24-96 h, respectively. These parameters were optimized using Response Surface Methodology (RSM) based Box-Behnken Design (BBD). It was found that a maximum ethanol concentration of 0.2224 g/ml was obtained from papaya waste at pH 4.5, 45°C and 24 hours. The significance of the parameters increased from incubation time, pH to temperature.
Keywords
Biofuel, Ethanol, Optimization, Response Surface Methodology
Published online 5/20/2023, 9 pages
Copyright © 2023 by the author(s)
Published under license by Materials Research Forum LLC., Millersville PA, USA
Citation: TAN Ching Mun, OH Pei Ching, Optimization of bioethanol production from papaya waste through fermentation using response surface methodology (RSM), Materials Research Proceedings, Vol. 29, pp 72-80, 2023
DOI: https://doi.org/10.21741/9781644902516-10
The article was published as article 10 of the book Sustainable Processes and Clean Energy Transition
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 license. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.
References
[1] “bp Statistical Review of World Energy 2020,” in “Statistical Review of World Energy,” bp, 2020, vol. 69. [Online]. Available: sr@bp.com
[2] Z. H. Zhao, “Design Principle on Carbon Nanomaterials Electrocatalysts for Energy Storage and Conversion,” p. 134, 2017, https://doi.org/10.13140/RG.2.2.25704.16641
[3] S. Nikolic, V. Lazic, D. Veljovic, and L. Mojovic, “Production of bioethanol from pre-treated cotton fabrics and waste cotton materials,” Carbohydrate Polymers, vol. 164, pp. 136-144, May 15 2017, https://doi.org/10.1016/j.carbpol.2017.01.090
[4] O. Oiwoh, B. V. Ayodele, N. A. Amenaghawon, and C. O. Okieimen, “Optimization of bioethanol production from simultaneous saccharification and fermentation of pineapple eels using Saccharomyces cerevisiae,” Journal of Applied Sciences and Environmental Management, vol. 22, no. 1, 2018, https://doi.org/10.4314/jasem.v22i1.10
[5] N. Annamalai, H. Al Battashi, S. N. Anu, A. Al Azkawi, S. Al Bahry, and N. Sivakumar, “Enhanced Bioethanol Production from Waste Paper Through Separate Hydrolysis and Fermentation,” Waste and Biomass Valorization, vol. 11, no. 1, pp. 121-131, 2018, https://doi.org/10.1007/s12649-018-0400-0
[6] M. Bhuvaneswari and N. Sivakumar, “Bioethanol Production from Fruit and Vegetable Wastes,” Bioprocessing for Biomolecules Production, no. 1, pp. 417-427, 2020.
[7] A. Oliveira, “Market analysis for Papaya (Production and Trade),” 2018.
[8] C. Pavithra, S. S. Devi, J. S. W, and C. V. D. Rani, “Nutritional properties of papaya peel,” The Pharma Innovation Journal, vol. 6, no. 7, pp. 170-173, 2017.
[9] G. L. Utama, Tyagita, I. Krissanti, D. W. Wira, and R. L. Balia, “Stress Tolerance Yeast Strain from Papaya Wastes for Bioethanol Production,” International Journal of GEOMATE, vol. 17, no. 61, 2019, https://doi.org/10.21660/2019.61.4796
[10] N. A. A. Hamid and N. Z. Zulkifli, “Papaya peels as source of hydro char via hydrothermal carbonization,” IOP Conf. Series: Earth and Environmental Science, vol. 765, 2021.
[11] J. J. Romero, M. A. Zarate, I. M. Ogunade, K. G. Arriola, and A. T. Adesogan, “Tropical plant supplementation effects on the performance and parasite burden of goats,” Asian-Australas J Anim Sci, vol. 31, no. 2, pp. 208-217, Feb 2018, https://doi.org/10.5713/ajas.17.0321
[12] N. I. Wan Azelee, J. Md Jahim, A. Rabu, A. M. Abdul Murad, F. D. Abu Bakar, and R. Md Illias, “Efficient removal of lignin with the maintenance of hemicellulose from kenaf by two-stage pretreatment process,” Carbohydr Polym, vol. 99, pp. 447-53, Jan 2014, https://doi.org/10.1016/j.carbpol.2013.08.043
[13] R. Abdulla, E. Derman, P. T. Ravintaran, and S. A. Jambo, “Fuel Ethanol Production from Papaya Waste using Immobilized Saccharomyces cerevisiae,” ASM Science Journal, vol. 11, no. 2, pp. 112-123, 2018
[14] P. K. Dash, S. Mohaptra, M. R. Swain, and H. Thatoi, “Optimization of bioethanol production from saccharified sweet potato root flour by co-fermentation of Saccharomyces cerevisiae and Pichia sp. using OVAT and response surface methodologies,” Acta Biologica Szegediensis, vol. 61, pp. 13-23, 2017.
[15] K. Ma, Z. Ruan, Z. Shui, Y. Wang, G. Hu, and M. He, “Open Fermentative Production of Fuel Ethanol from Food Waste by An Acid-Tolerant Mutant Strain of Zymomonas Mobilis,” Bioresour Technol, vol. 203, pp. 295-302, Mar 2016, https://doi.org/10.1016/j.biortech.2015.12.054
[16] G. Reed and H. J. Peppler, “Yeast technology,” Food, vol. 19, no. 4, 1975, https://doi.org/10.1002/food.19750190413
[17] A. K. Pai, R. K. Vikas, H. S. Neeraja, C. A. Puneeth, and C. V. Rao, “Bioethanol Production from Waste Biomass,” India Patent Appl. 2373/CHE/2009 A, 2011.
[18] Y. Lugani et al., “Recent advances in bioethanol production from lignocelluloses: a comprehensive review with a focus on enzyme engineering and designer biocatalysts,” Biofuel Research Journal, vol. 7, no. 4, pp. 1267-1295, 2020, https://doi.org/10.18331/brj2020.7.4.5
[19] M. R. A. Ghani and P. C. Oh, “Optimization of Ethanol Production from Mango Peels Using Response Surface Methodology,” Applied Mechanics and Materials, vol. 625, pp. 766-769, 2014, https://doi.org/10.4028/www.scientific.net/AMM.625.766
[20] S. Chongkhong, B. Lolharat, and P. Chetpattananondh, “Optimization of Ethanol Production from Fresh Jackfruit Seeds Using Response Surface Methodology,” Journal of Sustainable Energy & Environment, vol. 3, pp. 97-101, 2012.
[21] D. Dahnum, S. O. Tasum, E. Triwahyuni, M. Nurdin, and H. Abimanyu, “Comparison of SHF and SSF Process Using Enzyme and Dry Yeast for Optimization of Bioethanol Production from Empty Fruit Bunch,” Energy Procedia, vol 68, pp.107-116, 2015, https://doi.org/10.1016/j.egypro.2015.03.238
[22] A. M. Saeed, H. Ma, S. Yue, Q. Wang, and M. Tu, “Concise review on ethanol production from food waste: development and sustainability,” Environ Sci Pollut Res Int, vol. 25, no. 29, pp. 28851-28863, Oct 2018, https://doi.org/10.1007/s11356-018-2972-4
