Nanoemulsions: Preparation, Properties and Applications
Preeti Gupta, Vinita, S.S. Das
In the last few decades, Nanoemulsions (NE) have gained significant interest among researchers because of their improved functional properties in comparison to emulsions. These include significant properties such as optical and rheological properties, coalescence, flocculation etc. It also shows excellent kinetic and thermodynamic stability. These NEs have been used in a variety of applications such as food, cosmetic and oil industries, preservatives, antimicrobial agent, in different drug delivery systems, cell culture technology etc. The chapter focuses on the various synthesis methods of Nanoemulsions. This will also provide insight about the important and useful properties. Applications in a variety of disciplines have also been discussed in detail.
Keywords
Nanoemulsion, Drug Delivery, Stability, Cosmetic Industry, Ultrasonication
Published online 11/15/2022, 26 pages
Citation: Preeti Gupta, Vinita, S.S. Das, Nanoemulsions: Preparation, Properties and Applications, Materials Research Foundations, Vol. 135, pp 200-225, 2023
DOI: https://doi.org/10.21741/9781644902172-9
Part of the book on Emerging Nanomaterials and Their Impact on Society in the 21st Century
References
[1] J. Ashaolu, Nanoemulsions for health, food, and cosmetics: a review, Tolulope, Environ. Chem. Lett., 19 (2021),3381-3395. https://doi.org/10.1007/s10311-021-01216-9. https://doi.org/10.1007/s10311-021-01216-9
[2] P. Chowdhury, M. Gogoi, S. Borchetia et al, Nanotechnology applications and intellectual property rights in agriculture. Environ Chem Lett 15(3) (2017) 413-419. https:// doi. org/ 10. 1007/s10311- 017- 0632-4. https://doi.org/10.1007/s10311-017-0632-4
[3] A. M. Rodríguez , A. T.Ibarz , L. S.Trujillo , O. M. Belloso, Incorporation of antimicrobial nanoemulsions into complex foods: A case study in an apple juice-based beverage, LWT-Food Sci Tech., 141 (2021) 110926. https://doi.org/10.1016/j.lwt.2021.110926
[4] J. S. Franklynea , P. M. Gopinatha , A. Mukherjee, N. Chandrasekaran, Nanoemulsions: The rising star of antiviral therapeutics and nanodelivery system-current status and prospects, Current Opinion Colloid & Inter. Sci., 2021, 54:101458. https://doi.org/10.1016/j.cocis.2021.101458
[5] S. M. El-Sayed, H. S. El-Sayed, Antimicrobial nanoemulsion formulation based on thyme (Thymus vulgaris) essential oil for UF labneh preservation journal of materials research and technology,;10 (2021)1029-1041. https://doi.org/10.1016/j.jmrt.2020.12.073
[6] P. Norouzia, A. Rastegarib, F. Mottaghitalabc , M. Farokhid , P.Zarrintaje , M.R.Saeb, Nanoemulsions for intravenous drug delivery in: Nanoengineered Biomaterials for Advanced Drug Delivery, Elsevier Ltd (2020). https://doi.org/10.1016/B978-0-08-102985-5.00024-3. https://doi.org/10.1016/B978-0-08-102985-5.00024-3
[7] M.L. Zambrano-Zaragoza, D. Quintanar-Guerrero, N. Mendoza-Mun˜oz, G. Leyva-Go’mez, Nanoemulsions and nanosized ingredients for food formulations in: Handbook of Food Nanotechnology Elsevier ltd. (2020). DOI: https://doi.org/10.1016/B978-0-12-815866-1.00006-6 ©. https://doi.org/10.1016/B978-0-12-815866-1.00006-6
[8] S.A. Chime, F.C. Kenechukwu and A.A. Attama, Nanoemulsions – Advances in Formulation, Characterization and Applications in Drug Delivery, in: A.D.Sezer (Ed.), Applications in Drug Delivery, Intech Open, London. 2014. https://doi.org/10.5772/58673
[9] A.C. Faria-Silva, A. M. Costa, A. Ascenso, H. M. Ribeiro, J. Marto, L. Maria Gonc¸ alves, M. Carvalheiro, S.Simo˜es, Nanoemulsions for cosmetic products, Nanocosmetics Fundamen.Appl. Toxic. Micro Nano Techn., (2020) 59-77 https://doi.org/10.1016/B978-0-12-822286-7.00004-8
[10] V.Pandey, R. Shukla, A. Garg, M.L.Kori, G.Rai, Nanoemulsions for cosmetic products: From laborartory to market, DOI: https://doi.org/10.1016/B978-0-12-822286-7.00004-8 2020 Elsevier Inc. https://doi.org/10.1016/B978-0-12-822286-7.00021-8
[11] R. K. Harwansha, R. Deshmukha , Md Akhlaquer Rahman , Nanoemulsion: Promising nanocarrier system for delivery of herbal bioactives Journal of Drug Delivery Science and Technology 51 (2019) 224-233 https://doi.org/10.1016/j.jddst.2019.03.006
[12] Z. Karami, M. R. S. Zanjani, M. Hamidi, Nanoemulsions in CNS drug delivery: recent developments, impacts and challenges, Drug Discovery Today, 24, (2019) 1104-1115. https://doi.org/10.1016/j.drudis.2019.03.021
[13] N. Kumar, A. Verma, A. M. Formation, Characteristics and oil industry applications of nanoemulsions: A review, J. Petro. Sci. Eng., 206, (2021) 109042. https://doi.org/10.1016/j.petrol.2021.109042
[14] Y.Singh, J.G.Meher, K..Raval, F. A.Khan, M. Chaurasia, N. K. Jain, M. K. Chourasia, Nanoemulsion: Concepts, development and applications in drug delivery, J. Controlled Release, 252 (2017) 28-49. https://doi.org/10.1016/j.jconrel.2017.03.008
[15] N. M. Aljabri, N. Shi, A.Cavazos, Nanoemulsion: An emerging technology for oilfield applications between limitations and potentials, J. Petroleum Sci. Engg., 208 (2022) 109306. https://doi.org/10.1016/j.petrol.2021.109306
[16] M. M. Badr, M. E.I. Badawy, N. E.M. Taktak, Characterization, antimicrobial activity, and antioxidant activity of the nanoemulsions of Lavandula spica essential oil and its main monoterpenes, J. Drug Delivery Sci. Tech., 65 (2021) 102732. https://doi.org/10.1016/j.jddst.2021.102732
[17] L. Almasi, M. Radi, S. Amiri, D. J. McClements, Fabrication and characterization of antimicrobial biopolymer films containing essential oil-loaded microemulsions or nanoemulsions, Food Hydrocolloids, 117 (2021) 106733. https://doi.org/10.1016/j.foodhyd.2021.106733
[18] D. J. McClements, Advances in edible nanoemulsions: Digestion, bioavailability, and potential toxicity, Progress in Lipid Res,, 81 (2021) 101081. https://doi.org/10.1016/j.plipres.2020.101081
[19] P. Nirale, A. Paul, K. S. Yadav, Nanoemulsions for targeting the neurodegenerative diseases: Alzheimer’s, Parkinson’s and Prion’s, Life Sci., 245 (2020) 117394. https://doi.org/10.1016/j.lfs.2020.117394
[20] Y. Ozogula, E. K. Boğaa, I. Akyolb , M.Durmusa , Y. Ucarc , J. M. Regensteind , A.R. Köşker, Antimicrobial activity of thyme essential oil nanoemulsions on spoilage bacteria of fish and food-borne pathogens, Food Biosci., 36 (2020) 10063. https://doi.org/10.1016/j.fbio.2020.100635
[21] J. B.Aswathanarayan, R. R. Vittal, Nanoemulsions and Their Potential Applications in Food Industry, Frontiers in sustainable Food systems, 3 (2019) 1-21. https://doi.org/10.3389/fsufs.2019.00095
[22] A. Saxena, T.Maity, A.Paliwal, S. Wadhwa, Technological aspects of nanoemulsions and their applications in the food sector, in: Nanotechnology Applications in Food, Cambridge, MA: Academic Press), (2017). 129- 152. https://doi.org/10.1016/B978-0-12-811942-6.00007-8
[23] D. J. McClements, J. Rao, Food-grade nanoemulsions: formulation, fabrication, properties, performance, biological fate, and potential toxicity., Crit. Rev. Food Sci. Nutr., 51 (2011). 285-330. https://doi.org/10.1080/10408398.2011.559558
[24] S. Kaul, N. Gulati, D. Verma, S. Mukherjee, U. Nagaich, Role of nanotechnology in cosmeceuticals: a review of recent advances. J Pharm, (2018) 3420204. https://doi.org/10.1155/2018/3420204. https://doi.org/10.1155/2018/3420204
[25] Feng J, Zhang Q, Liu Q et al (2018) Application of nanoemulsions in formulation of pesticides. In:Nanoemulsions. Academic Press, pp 379-413. https://doi.org/10.1016/B978-0-12-811838-2. 00012-6 https://doi.org/10.1016/B978-0-12-811838-2.00012-6
[26] ZAA Aziz, Mohd- H Nasir, A. Ahmad, Mohd.S. H.Setapar, W. L. Peng4, S. C.Chuo, A. Khatoon, K. Umar, A. A. Yaqoob, Mohd. N. Mohamad Ibrahim, Role of nanotechnology for design and development of cosmeceutical: application in makeup and skin care. Front Chem 7 (2019) 739. https://doi.org/10.3389/fchem.2019.00739
[27] S. Sharma, M. Hegde, V. Sadananda, B. Matthews, In vitro study to evaluate laser fluorescence device for monitoring the effect of aluminum gallium arsenide laser on noncavitated enamel lesions, J. Dent. Lasers, 11 (2017) 26. https://doi.org/10.4103/jdl.jdl_17_16
[28] S., Talegaonkar, L.M., Negi, Nanoemulsion in drug targeting. In: Devarajan, P.V., Jain, S. (Eds.), Targeted Drug Delivery: Concepts and Design. Springer International Publishing, Cham, 2015, pp. 433459. https://doi.org/10.1007/978-3-319-11355-5_14
[29] N. Bainun, N. Hashimah, S. Shatir, S. Hassan, Nanoemulsion: Formation, Characterization, Properties and Applications- A review, Adv. Mater. Res., 1113 (2015) 147-152. https://doi.org/10.4028/www.scientific.net/AMR.1113.147
[30] M. Quintanilla-Carvajal, B. Camacho-Diaz, L. Meraz-Torres, J. Chanona-Perez, L. Alamilla -Beltran, A. Jimenez-Aparicio, et al., Nanoencapsulation: A new trend in food engineering processing, Food Eng. Rev. 2 (2010) 39-50., https://doi.org/10.1007/s12393-009-9012-6
[31] S. M. Afari, E. Assadpoor, Y. H. He, and B. Bhandari, Re-coalescence of emulsion droplets during high-energy emulsification, Food Hydrocoll. 22 (2008) 1191-1202. doi: 10.1016/j.foodhyd.2007.09.006 https://doi.org/10.1016/j.foodhyd.2007.09.006
[32] S. J. Lee, and D. J. McClements, Fabrication of protein-stabilized nanoemulsions using a combined homogenization and amphiphilic solvent dissolution/evaporation approach, Food Hydrocoll. 24 (2010) 560-569. doi: 10.1016/j.foodhyd.2010.02.002. https://doi.org/10.1016/j.foodhyd.2010.02.002
[33] R. Cappellani, M. ,Perinelli, D. Romano, P. Laura, S. Aurélie C. Marco , C. Riccardo, B. Paolo, Injectable nanoemulsions prepared by high pressure homogenization: processing, sterilization, and size evolution, Appl. Nanosci. 8 (6) (2018) 1483-14919 https://doi.org/10.1007/s13204-018-0829-2
[34] H. Schubert, and R. Engel, Product and formulation engineering of emulsions. Chem. Eng. Res. Des. 82 (2004) 1137-1143. doi: 10.1205/cerd.82.9.1137.44154Liu et al., 2019 https://doi.org/10.1205/cerd.82.9.1137.44154
[35] R. Severino, G. Ferrari, K. D. Vu, F. Donsi, S. Salmieri, and M. Lacroix, Antimicrobial effects of modified chitosan based coating containing nanoemulsion of essential oils, modified atmosphere packaging and gamma irradiation against Escherichia coli O157:H7 and Salmonella Typhimurium on green beans, Food Control, 50 (2015) 215-222. doi: 10.1016/j.foodcont.2014 https://doi.org/10.1016/j.foodcont.2014.08.029
[36] Y. N. Shariffa, T. B. Tan, U. Uthumporn, F.Abas, H. Mirhosseini, I. A. Nehdi, et al. Producing a lycopene nanodispersion: formulation development and the effects of high pressure homogenization, Food Res. Int. 101 (2017) 165-172. https://doi.org/10.1016/j.foodres.2017.09.005
a. doi: 10.1016/j.foodres.2017.09.005
[37] A. Håkansson, Can high-pressure homogenization cause thermal degradation to nutrients? J. Food Eng. 240 (2019) 133-144. https://doi.org/10.1016/j.jfoodeng.2018.07.024
a. doi: 10.1016/j.jfoodeng.2018.07.024
[38] K. Nakabayashi, F. Amemiya, T. Fuchigami, K. Machida, S. Takeda, K. Tamamitsub, et al. Highly clear and transparent nanoemulsion preparation under surfactant-free conditions using tandem acoustic emulsification. Chem. Commun. 47 (2011) 5765-5767. doi: 10.1039/c1cc10558b https://doi.org/10.1039/c1cc10558b
[39] N. Anton, and T. F. Vandamme, Nano-emulsions and microemulsions: clarifications of the critical differences, Pharm. Res. 28 (2011) 978-985. doi: 10.1007/s11095-010-0309-1 https://doi.org/10.1007/s11095-010-0309-1
[40] R. K. Harwansha, R. Deshmukha, M. A. Rahman, Nanoemulsion: Promising nanocarrier system for delivery of herbal bioactives, J. Drug Delivery Sci. Tech., 51 (2019) 224 -233. https://doi.org/10.1016/j.jddst.2019.03.006
[41] L. Wang, X. Li, G. Zhang, J. Dong, J. Eastoe, Oil-in-water nanoemulsions for pesticide formulations, J. Colloid Interface Sci. 314 (2007) 230-235. https://doi.org/10.1016/j.jcis.2007.04.079
[42] S. Lamaallam, H. Bataller, C. Dicharry, J. Lachaise, Formation and stability of miniemulsions produced by dispersion of water/oil/surfactants concentrates in a large amount of water, Colloid. Surf. Physicochem. Eng. Asp. 270 (2005) 44-51. https://doi.org/10.1016/j.colsurfa.2005.05.035
[43] C. Solans, J. Esquena, A.M. Forgiarini, N. Uson, D. Morales, P. Izquierdo, Nanoemulsions: formation and properties, in Solution: Fundamentals and Applications, Surfactants Marcel Dekker, New York, (2002) 525-554. https://doi.org/10.1201/9780203910573.ch25
[44] J. C. L. Liew, Q. D. Nguyen, and Y. Ngothai, Effect of sodium chloride on the formation and stability of n-dodecane nanoemulsions by the PIT method. Asia Pac. J. Chem. Eng. 5 (2010) 570-576. doi: 10.1002/apj.445. https://doi.org/10.1002/apj.445
[45] G. R. Deen, J. Skovgaard, J. S. Pedersen, FORMATION AND PROPERTIES OF NANOEMULSIONS, Emulsions, (2016) 193-226. https://dx.doi.org/10.1016/B978-0-12-804306-6.00006-4. https://doi.org/10.1016/B978-0-12-804306-6.00006-4
[46] A. D. Gadhave, Nanoemulsions: Formation, Stability and Applications International Journal for Research in Science & Advanced Technologies Issue-3, Volume-2 (2014) 038-043, 2014.
[47] G. P. Malode, S. A. Chavhan, S. A. Bartare, L. L. Malode, J. V. Manwar, R. L. Baka, A Critical Review on Nanoemulsion: Advantages, Techniques and Characterization Journal of Applied Pharmaceutical Sciences and Research, 4(3) (2021). https://doi.org/10.31069/japsr.v4i3.2
[48] Development and characterization of nanoemulsion as carrier for the enhancement of bioavailability of artemether Moksha Laxmi, Ankur Bhardwaj, Shuchi Mehta & Abhinav Mehta, Artificial Cells, Nanomedicine, and Biotechnology, 43 (2015) 334-344. https://doi.org/10.3109/21691401.2014.887018
[49] D. J. McClements, Fabrication, characterization and properties of food, nano emulsions, University of Massachusetts, USA, Woodhead Publishing Limited, (2012). https://doi.org/10.1533/9780857095657.2.293
[50] W. Jin, W. Xu, H. Liang, Y. Li, S. Liu, B. Li, NANOEMULSIONS FOR FOOD: PROPERTIES, PRODUCTION, CHARACTERIZATION, AND APPLICATIONS, Emulsions. (2016) https://dx.doi.org/10.1016/B978-0-12-804306-6.00001. https://doi.org/10.1016/B978-0-12-804306-6.00001-5
[51] G. R. Deen, J. Skovgaard, J. S. Pedersen, FORMATION AND PROPERTIES OF NANOEMULSIONS, Emulsions, (2016) https://dx.doi.org/10.1016/B978-0-12-804306-6.00006-4. https://doi.org/10.1016/B978-0-12-804306-6.00006-4
[52] Z. Zhang and D. J. McClements, Overview of Nanoemulsion Properties: Stability, Rheology, and Appearance, Nanoemulsions (2018) https://doi.org/10.1016/B978-0-12- https://doi.org/10.1016/B978-0-12-811838-2.00002-3
[53] D.J. McClements, Food Emulsions: Principles, Practices, and Techniques. CRC Press, Boca Raton, FL. (2015). https://doi.org/10.1201/b18868
[54] A.S. Kabalnov, E.D. Shchukin, Ostwald ripening theory: applications to fluorocarbon emulsion stability. Adv. Colloid Interf. Sci. 38 (1992) 69-97. https://doi.org/10.1016/0001-8686(92)80043-W
[55] J. Chen, J.R. Stokes, Rheology and tribology: two distinctive regimes of food texture sensation. Trends Food Sci. Technol. 25 (2012) 4-12. https://doi.org/10.1016/j.tifs.2011.11.006
[56] D.J. McClements, Emulsion design to improve the delivery of functional lipophilic components. Annu. Rev. Food Sci. Technol. 1 (2010) 241-269. https://doi.org/10.1146/annurev.food.080708.100722
[57] R.J. Hunter, Foundations of Colloid Science. Oxford University Press, New York, NY (2001).
[58] K. Fuentes, C. Matamala, N. Martínez, R. N. Zúñiga, and E. Troncoso, Comparative Study of Physicochemical Properties of Nanoemulsions Fabricated with Natural and Synthetic Surfactants Processes 9 (2021) 2002. https://doi.org/10.3390/pr9112002. https://doi.org/10.3390/pr9112002
[59] T.J. Wooster, D. Labbett, P. Sanguansri, H. Andrews, Impact of microemulsion inspired approaches on the formation and destabilisation mechanisms of triglyceride nanoemulsions. Soft Matter 12 (2016) 1425-1435. https://doi.org/10.1039/C5SM02303C
[60] K. GURPREET AND S. K. SINGH, Review of Nanoemulsion Formulation and Characterization Techniques, Indian Journal of Pharmaceutical Science, J Pharm Sci 80(5) (2018) 781-789. https://doi.org/10.4172/pharmaceutical-sciences.1000422
[61] P. J. P. Espitia , C. A. Fuenmayor , and C. G. Otoni, Nanoemulsions: Synthesis, Characterization, and Application in Bio-Based Active Food Packaging, Comprehensive Reviews in Food Science and Food Safety, (2018) doi: 10.1111/1541-4337.12405. https://doi.org/10.1111/1541-4337.12405
[62] N. Robledo, L. López, A. Bunger, et al. Effects of antimicrobial edible coating of thymol nanoemulsion/quinoa protein/chitosan on the safety, sensorial properties, and quality of refrigerated strawberries (Fragaria × ananassa) under commercial storage environment. Food and Bioprocess Technology, 11(8) (2008) 1566-1574. https://doi.org/10.1007/s11947-018-2124-3, https://doi.org/10.1007/s11947-018-2124-3
[63] Y. A. Oh, Y. J. Oh, A. Y. Song, J. S. Won, K. B. Song, & S. C. Min, Comparison of effectiveness of edible coatings using emulsions containing lemongrass oil of different size droplets on grape berry safety and preservation. LWT, 75 (2017) 742-750. https://doi.org/10.1016/j.lwt.2016.10.033, https://doi.org/10.1016/j.lwt.2016.10.033
[64] I. Dammak, R. A. d. Carvalho, C. S. F. Trindade, R. V. Lourenc¸ P. J. d. A. Sobral, Properties of active gelatin films incorporated with rutin-loaded nanoemulsions, International Journal of Biological Macromolecules 98 (2017) 39-49. https://doi.org/10.1016/j.ijbiomac.2017.01.094
[65] E. M. C. Alexandre1, R. V. Lourenço, A. M. Q. B. Bittante, I. C. F. Moraes, P. J. d. A. Sobral, Gelatin-based films reinforced with montmorillonite and activated with nanoemulsion of ginger essential oil for food packaging applications , Food Packaging and Shelf Life 10 (2016) 87-96 . https://doi.org/10.1016/j.fpsl.2016.10.004
[66] K. Bhargava, D. S. Conti, S. R. P. da Rocha, and Y. Zhang, Application of an oregano oil nanoemulsion to the control of foodborne bacteria on fresh lettuce. Food Microbiol. 47 (2015) 69-73. doi: 10.1016/j.fm.2014.11.007. https://doi.org/10.1016/j.fm.2014.11.007
[67] S. Goindi, A. Kaur, R. Kaur, A. Kalra, and P. Chauhan, Nanoemulsions: an emerging technology in the food industry. Emulsions. 3 (2016) 651-688. doi: 10.1016/B978-0-12-804306-6.00019-2. https://doi.org/10.1016/B978-0-12-804306-6.00019-2
[68] Z. Wei, and Y. Gao, Physicochemical properties of β-carotene bilayer emulsions coated by milk proteins and chitosan-EGCG conjugates. Food Hydrocoll. 52 (2016) 590-599. doi: 10.1016/j.foodhyd.2015.08.002. https://doi.org/10.1016/j.foodhyd.2015.08.002
[69] E. M. C. Alexandre, R. V. Lourenço, A. M. Q. B. Bittante, I. C. F. Moraes, and P. J. A. Sobral, Gelatine based films reinforced with montmorillonite and activated with nanoemulsion of ginger essential oil for food packaging applications. Food Pack. Shelf Life. 10 (2016) 87-96. doi: 10.1016/j.fpsl.2016.10.004. https://doi.org/10.1016/j.fpsl.2016.10.004
[70] C. G. Otoni, M. R. de Moura, F. A. Aouada, G. P. Camilloto, R. S. Cruz, M. V. Lorevice, et al. Antimicrobial and physicalmechanical properties of pectin/papaya puree/cinnamaldehyde nanoemulsion edible composite films, Food Hydrocoll. 41 (2014) 188-194. doi: 10.1016/j.foodhyd.2014.04.013 . https://doi.org/10.1016/j.foodhyd.2014.04.013
[71] S. Sugumar, S. Singh, A. Mukherjee, and N. Chandrasekaran, Nanoemulsion of orange oil with non ionic surfactant produced emulsion using ultrasonication technique: evaluating against food spoilage yeast. Appl. Nanosci. 6 (2015) 113-120. doi: 10.1007/s13204-015-0412-. https://doi.org/10.1007/s13204-015-0412-z
[72] C. G. Otoni, S. F. Pontes, E. A., Medeiros, and N. F. Soares, Edible films from methylcellulose and nanoemulsions of clovebud (Syzygium aromaticum) and oregano (Origanum vulgare) essential oils as shelf life extenders for sliced bread. J. Agric. Food Chem. 62 (2014) 5214-5219. doi: 10.1021/jf501055f. https://doi.org/10.1021/jf501055f
[73] J. Meyer, G. Polak, R. Scheuermann, Preparing PIC emulsions with a very fine particle size. Cosmet Toilet, 122 (2007) 6170.
[74] P. Heunnemann, S. Pre’vost, I. Grillo, C.M. Marino, J. Meyer, M. Gradzielski, Formation and structure of slightly anionically charged nanoemulsions obtained by the phase inversion concentration (PIC) method. Soft Matter 7(12) (2011) 5697710. https://doi.org/10.1039/C0SM01556C. https://doi.org/10.1039/c0sm01556c
[75] Sinerga. NANOCREAMs-Fine particle-size emulsifier, ,https://www.sinerga.it/files/materie-prime/ nanocream/nanocream-flyer.pdf.; [accessed 16.10. 19].
[76] Z, Hu M, Liao Y, Chen Y, Cai L, Meng Y, Liu et al. A novel preparation method for silicone oil nanoemulsions and its application for coating hair with silicone, Int J Nanomedicine.,7 (2012) 5719-5724. https://doi.org/10.2147/IJN.S37277
[77] K.H. Ranjit, C.P. Kartik, K.P. Surendra, S. Jagadish, A.R. Mohammed, Nanoemulsions as vehicles for transdermal delivery of glycyrrhizin. Braz. J. Pharm. Sci. 47(4) (2011) 13-19. https://doi.org/10.1590/S1984-82502011000400014
[78] D. Mou, H. Chen, D. Du, C. Mao, J. Wan, H. Xu, X. Yang, Hydrogel-thickened nanoemulsion system for topical delivery of lipophilic drugs, Int. J. Pharm. 353 (2008) 270-276. https://doi.org/10.1016/j.ijpharm.2007.11.051
[79] O.A., Hussein, H.A. Salama, M. Ghorab, A.A. Mahmoud, Nanoemulsion as a po‐ tential ophthalmic delivery system for dorzolamide hydrochloride. AAPS PharmSci‐ Tech. 10(3) (2009) 808-819. https://doi.org/10.1208/s12249-009-9268-4
[80] C. Chircov and A. M. Grumezescu, Nanoemulsion preparation, characterization, and application in the field of biomedicine, Nanoarchitectonics in Biomedicine. (2019) DOI: https://doi.org/10.1016/B978-0-12-816200-2.00019-0 https://doi.org/10.1016/B978-0-12-816200-2.00019-0
[81] M. Gallarate, D. Chirio, R. Bussano, E. Peira, L. Battaglia, F. Baratta, et al., Development of O/W nanoemulsions for ophthalmic administration of timolol. Int. J. Pharm. 440 (2) (2013) 126134. https://doi.org/10.1016/j.ijpharm.2012.10.015
[82] J.C. Schwarz, V. Klang, S. Karall, D. Mahrhauser, G.P. Resch, C. Valenta, Optimisation of multiple W/O/W nanoemulsions for dermal delivery of aciclovir. Int. J. Pharm. 435 (1) (2012) 6975. https://doi.org/10.1016/j.ijpharm.2011.11.038
[83] H.L. Alvarado, G. Abrego, E.B. Souto, M.L. Gardun˜o-Ramirez, B. Clares, M. L. Garcı’a, et al., Nanoemulsions for dermal controlled release of oleanolic and ursolic acids: in vitro, ex vivo and in vivo characterization. Colloids Surf., B: Biointerfaces 130 (2015) 4047. https://doi.org/10.1016/j.colsurfb.2015.03.062
[84] T. Isailovic, S. Ðorðevi, B. Markovi D. Ranðelovi N. Ceki M. Luki, et al., Biocompatible nanoemulsions for improved aceclofenac skin delivery: formulation approach using combined mixture-process experimental design. J. Pharm. Sci. 105 (1) (2016) 308323. https://doi.org/10.1002/jps.24706
[85] L. Sosa, B. Clares, H.L. Alvarado, N. Bozal, O. Domenech, A.C. Calpena, Amphotericin B releasing topical nanoemulsion for the treatment of candidiasis and aspergillosis. Nanomed. Nanotechnol. Biol. Med. 13 (7) (2017) 23032312. https://doi.org/10.1016/j.nano.2017.06.021
[86] M. Aqil, M. Kamran,A. Ahad, S.S. Imam, Development of clove oil based nanoemulsion of olmesartan for transdermal delivery: BoxBehnken design optimization and pharmacokinetic evaluation. J. Mol. Liq. 214 (2016) 238248 https://doi.org/10.1016/j.molliq.2015.12.077
[87] F. Shakeel, W. Ramadan, Transdermal delivery of anticancer drug caffeine from water-in-oil nanoemulsions. Colloids Surf., B: Biointerfaces 75 (1) (2010) 356362. https://doi.org/10.1016/j.colsurfb.2009.09.010
[88] Y. Gao, X. Cheng, Z. Wang, J. Wang, T. Gao, P. Li, et al., Transdermal delivery of 10,11-methylenedioxycamptothecin by hyaluronic acid based nanoemulsion for inhibition of keloid fibroblast. Carbohydr. Polym. 112 (2014) 376386. https://doi.org/10.1016/j.carbpol.2014.05.026
[89] I. Tamayo, C. Gamazo, J. de Souza Rebouc, J.M. Irache, Topical immunization using a nanoemulsion containing bacterial membrane antigens. J. Drug Delivery Sci. Technol. 42 (2017) 207214. https://doi.org/10.1016/j.jddst.2017.02.009
[90] G. Ahmad, R. El Sadda, G. Botchkina, I. Ojima, J. Egan, M. Amiji, Nanoemulsion formulation of a novel taxoid DHA-SBT-1214 inhibits prostate cancer stem cell-induced tumor growth. Cancer Lett. 406 (2017) 7180. https://doi.org/10.1016/j.canlet.2017.08.004
[91] Leandro, F.Z., Martins, J., Fontes, A.M., Tedesco, A.C., 2017. Evaluation of theranostic nanocarriers for near-infrared imaging and photodynamic therapy on human prostate cancer cells. Colloids Surf., B: Biointerfaces 154, 341349. https://doi.org/10.1016/j.colsurfb.2017.03.042
[92] L.P. Franchi, C.F Amantino, M.T. Melo, A.P. de Lima Montaldi, F.L. Primo, A.C. Tedesco, In vitro effects of photodynamic therapy induced by chloroaluminum phthalocyanine nanoemulsion. Photodiagn. Photodyn. Ther. 16 (2016) 100105. https://doi.org/10.1016/j.pdpdt.2016.09.003
[93] S. Natesan, A. Sugumaran, C. Ponnusamy, V. Thiagarajan, R. Palanichamy, R. Kandasamy, Chitosan stabilized camptothecin nanoemulsions: development, evaluation and biodistribution in preclinical breast cancer animal mode. Int. J. Biol. Macromol. 104 (2017) 18461852. https://doi.org/10.1016/j.ijbiomac.2017.05.127
[94] A.L. Silva, H.R. Marcelino, L.M. Verissimo, I.B. Araujo, L.F. Agnez-Lima, E.S. do Egito, Stearylamine-containing cationic nanoemulsion as a promising carrier for gene delivery. J. Nanosci. Nanotechnol. 16 (2) (2016) 13391345. https://doi.org/10.1166/jnn.2016.11671
[95] I. F. Mustafa and M. Z. Hussein, Synthesis and Technology of Nanoemulsion-Based Pesticide Formulation Nanomaterials 10 (2020) 1608. https://doi.org/10.3390/nano10081608
