Effect of some Micro-Elements on Steroids Production from Embryogenic Callus of in vitro Date Palm Sakkoty and Bartamuda Cultivars

Effect of some Micro-Elements on Steroids Production from Embryogenic Callus of in vitro Date Palm Sakkoty and Bartamuda Cultivars

Sherif F. El sharabasy, Abdel-Aal W. B., Hussein A. Bosila, Bayome M. Mansour, Abdel-Monem A. Bana

Abstract. The ability of plant cell, tissue, and organ cultures to produce and accumulate many of the same valuable chemical compounds as the parent plant in nature has been known almost since the inception of in vitro technology. Date palm has been recognized as an important crop containing high valuable secondary metabolism. Some microelements such as, manganese sulfate (MnSO42H2O), zinc sulfate (ZnSO47H2O) and copper sulfate (CuSO45H2O) were used as precursor to produce steroids from embryonic callus two date palm dry cvs. In this study, embryogenic callus explants were cultured on MS nutrient medium supplemented with different concentrations of ( MnSO42H2O), (22.3, 44.6 and 66.9 mg/l), ZnSO47H2O (8.6, 17.2 and 25.8 mg/l) and CuSO45H2O (0.025, 0.050, 0.075 mg/l). The highest significant value of total steroids ( 0.94 mg/g dry weight) was recorded when embryogenic callus of Sakkoty cv. was cultured on medium contained (22.3mg/l) MnSO42H2O. Where embryogenic callus of Bartamuda cv. cultured on nutrient medium supplemented at (17.2 mg/l) ZnSO47H2O gave the highest significant value of total steroid (0.92 mg/g dry weight).

Keywords
micro-elements, steroids, embryogenic callus, manganese sulfate, zinc sulfate, cupric sulfate, date palm cultivars

Published online 4/20/2019, 6 pages
Copyright © 2019 by the author(s)
Published under license by Materials Research Forum LLC., Millersville PA, USA

Citation: Sherif F. El sharabasy, Abdel-Aal W. B., Hussein A. Bosila, Bayome M. Mansour, Abdel-Monem A. Bana, Effect of some Micro-Elements on Steroids Production from Embryogenic Callus of in vitro Date Palm Sakkoty and Bartamuda Cultivars, Materials Research Proceedings, Vol. 11, pp 213-218, 2019

DOI: https://doi.org/10.21741/9781644900178-16

The article was published as article 16 of the book By-Products of Palm Trees and Their Applications

Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.

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